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Matches in Nanopublications for { ?s <http://purl.org/dc/elements/1.1/description> ?o ?g. }

• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "#!/usr/bin/env python3 import click @click.command() @click.option('--sample_input1') @click.option('--sample_input2') def step1(sample_input1, sample_input2): ''' description of step 1 ''' pass if __name__ == '__main__': step1()" assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "@FairStep(fw) def mult(walrus, bird): """ Multiply two integers together (walrus and bird). """ result = walrus * bird return result " assertion.
• step description "@FairStep(fw) def add(dog, cat): """ Add two integers together (dog and cat). """ result = dog + cat return result " assertion.
• step description "Incubate mycobacterial suspension at 80 °C for 1 h in a water bath. " assertion.
• step description "Add 500 μl phenol:chloroform:isoamyl alcohol (25:24:1) to the tube. Shake vigorously to mix thoroughly. Centrifuge the samples at 12,000 x g for 15 min." assertion.
• RAzPj2Eu9OhY55CH1kilJ-xWs2PJad7_3Fo-_JP8l8XsI.step description "To overcome this limitation, we have introduced a set of strategies for" RAzPj2Eu9OhY55CH1kilJ-xWs2PJad7_3Fo-_JP8l8XsI.assertion.
• RAEiCKIwbKY0YHZhMFWg-DxcaWOZZSs0dC6lzDyGkKM4Y.step description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." RAEiCKIwbKY0YHZhMFWg-DxcaWOZZSs0dC6lzDyGkKM4Y.assertion.
• RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ.step description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." RAFt6stRZSIiuA-w5go4rzFRQJci2Ez9yYnRw60sQMcYQ.assertion.
• RAHy1IDs_TuaRnFSah6lTrDNchlpBLc9_xHcevGrb1H2w.step description "Comments can take the form of short reviews, notes or questions to the author. Comments will be posted immediately. If reported by the community, we will temporarily hide the comment to determine if the comment is spam or harassment. If it is not, it will be unhidden, and it cannot be reported again. If you think a comment has been hidden or unhidden mistakenly, please contact us at" RAHy1IDs_TuaRnFSah6lTrDNchlpBLc9_xHcevGrb1H2w.assertion.
• RA43PHgfB-LOTUYXyIo5Ycb-NUESkJIgmcdtf_ocCJJdQ.step description "Wash worms off plates with M9 buffer and collect them in 15-ml Corning tubes." RA43PHgfB-LOTUYXyIo5Ycb-NUESkJIgmcdtf_ocCJJdQ.assertion.
• step description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." assertion.
• step description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." assertion.
• step description "Comments can take the form of short reviews, notes or questions to the author. Comments will be posted immediately. If reported by the community, we will temporarily hide the comment to determine if the comment is spam or harassment. If it is not, it will be unhidden, and it cannot be reported again. If you think a comment has been hidden or unhidden mistakenly, please contact us at" assertion.
• RAZFl-ZtaNMgJMxGNh6sPz4tFoy6ssHtz-kzqOsJWZGiI.step description "Wash worms off plates with M9 buffer and collect them in 15-ml Corning tubes." RAZFl-ZtaNMgJMxGNh6sPz4tFoy6ssHtz-kzqOsJWZGiI.assertion.
• step description "Cells can be kept in solution in the refrigerator for up to two hours." assertion.
• step description "Cells can be kept in solution in the refrigerator for up to two hours." assertion.
• step description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.
• step description "Aspirate the whitish buffy coat (about 1 ml) (PBMCs) formed in the interphase between histopaque and medium." assertion.
• step description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers." assertion.
• step description "Take 4 ml of Ficoll Histopaque in a 15 ml centrifuge tube." assertion.
• step description "Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates." assertion.
• step description "Discard medium and re-suspend the cell pellet in 1 ml of sterile Dulbecco's modified eagle medium." assertion.
• step description "Isolate human PBMCs by gradient centrifugation using Ficoll-Histophaque." assertion.
• step description "Eppendorf Safe-Lock microcentrifuge tube with tissue sample and glass ball (6 mm) freeze at -80°C, grind in the MM300 Mixer Mill for 2 min at 30 Hz. Alternatively, grind the sample in lysis solution." assertion.
• step description "Centrifuge at maximum speed for 2-5 min and carefully discard the supernatant by decanting or with a micropipette. A whitish DNA pellet should be visible during discarding a supernatant." assertion.
• step description " Discard supernatant and wash the pellet by adding 1.8 ml 70% ethanol, vortex thoroughly. At this stage, DNA samples can be stored at room temperature or refrigerated." assertion.
• step description "Spin at maximum speed in a microcentrifuge for 2 minutes." assertion.
• step description "The IBV-infected cells were incubated at 37 °C in 5% CO2" assertion.
• step description "At the indicated time points (0, 2, 4, 8, 12, 16, 20, 24, 28 h post-infection), cells were rinsed with 10 ml Phosphate Buffered Saline (PBS) buffer once and lysed in 1 ml TRIzol for 5 min at room temperature." assertion.
• step description "Shake tubes vigorously by hand for 15 sec and incubated for 3 min at room temperature, then centrifuged at 12,000 x g for 15 min at 4 °C. " assertion.
• step description "RNA was precipitated by centrifugation at 12,000 x g for 10 min at 4 °C." assertion.
• step description "The RNA pellets are air-dried and dissolved in 100 µl RNase-free H2O by incubating at 65 °C for 15 min" assertion.
• step description "The RNAs were stored at -80 °C for further use" assertion.
• step description "After incubating at room temperature for a few minutes, keep the solubilized nucleic acid in −20˚C for a short time storage or in −80˚C for a longtime storage. Figure 1 shows a schematic model of all the DNA and RNA isolation steps of this procedure." assertion.
• step description "Transfer the resulting solution to a sterile centrifuge tube (size=2 ml), and then mix sample by briefly vortexing until the sample is thoroughly resuspended." assertion.
• step description "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples." assertion.
• step description "After centrifuging at 5400 g at 4˚C for 5 min, remove the supernatant and then air-dry the pellet." assertion.
• step description "Centrifuge tubes at 13700 g at 4℃ for 10 min. The white pellet will be visible on the bottom of the tubes.CRITICAL STEP Do not disturb bottom phases of the solution when you pipet the upper phase." assertion.
• step description "Add 700 µl of cold isopropanol to precipitate RNA or DNA and then invert tubes 3-4 times to mix the solution." assertion.
• step description "Centrifuge at 13700 g at 4℃ for 10 min.CRITICAL STEP The PH of the extraction buffer is about 8-9, resulting in DNA and RNA precipitation, however, it could result in lower DNA and higher RNA concentrations in case of reducing the PH to around 6-7." assertion.
• step description "Grind mosquitoes in 1.5 ml eppendorf tube with micropistle in 50-100 µl 1X STE buffer ( 50mM Nacl,50mM Tris- HCL,100mM EDTA, Ph 8.0) along with 100mM sucrose. Add 1X STE buffer to a total volume of 300-500 µl for a single mosquito and 1 ml for mosquito pool like 4,6,8,10 numbers. Then add 1% SDS ,1% Triton -X , 10 µl/ ml Rnase A (20mg/ml), 20 µl/ ml Proteinase K (20mg/ml) and mix it." assertion.
• step description "Keep the pellet at 37 º celcius for 10 minutes." assertion.
• step description "Centrifuge at 12,000g for 30 minutes at 4º celcius and then remove the supernatant ." assertion.
• step description "Repeat the above step(5) , then add chloroform:isoamyl alcohol(24:1) and centrifuge at 12,000g for 10 minutes at 4º celcius ." assertion.
• step description "Centrifuge at 12,000g for 10 minutes at 4º celcius .Transfer the supernatant to a fresh tube." assertion.
• step description "After incubation, the tubes were centrifuged at 5000 × g for 10 min at 4°C and the supernatant was gently removed. The pellet is washed two times with 1ml of 70% ethanol and the DNA is pellet by 5000 × g at 4°C for only 5 min. The supernatant is discarded and the pellet is air-dried (10 min). The pellet are allowed to re-suspend in 50 μL of TE (10 mM Tris. HCl pH 8.0; 1 mM EDTA pH 8.0)." assertion.
• step description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions." assertion.
• step description "Centrifuge the tube for 10 min at 5000 × g at 4°C. Transfer the upper aqueous phase into new 2ml tubes and add 5μl RNAse A (10 mg/mL). Place the tube for 30 min at 37°C." assertion.
• step description "The falcon tube was kept into the 65°C incubator or water bath and mix gently by inversion after every 10 min till 45 min. After incubation, place the tube at room temperature for five min to reach to room temperature environment. Centrifuge the 50ml falcon tube for 5 min at 3000×g on room temperature. For next generation sequencing, the greater the genome size, lower is speed of initial centrifugation." assertion.
• step description "Add an equal volume of PCI mixture to the tube and mix contents by gently inverting tube for 5-10 min." assertion.
• step description "Snap freeze pellet in liquid N2 and immediately transfer to a mortar. Using a pestle, crush bacteria while repeatedly adding liquid N2 (~20 ml) to it." assertion.
• step description "Incubate mycobacterial suspension at 80 °C for 1 h in a water bath. This step will facilitate loosening of the mycobacterial cell wall and will also inactivate pathogenic mycobacteria." assertion.
• step description "Add an equal volume of isopropanol to tube. Mix contents gently and keep undisturbed for 5-10 min. The white thread-like structure of precipitated DNA will be seen in suspension" assertion.
• step description "Wash DNA pellet by adding 10 ml of 75% ethanol. Centrifuge at 12,000 x g for 10 min, 4 °C." assertion.
• step description "Add 100 µl 3 M sodium acetate solution per ml of aqueous phase obtained in step 10. Mix gently by inverting tube." assertion.
• step description "Centrifuge suspension at 12,000 x g for 10 min, 4 °C. Carefully transfer upper aqueous phase to a new 50 ml tube." assertion.

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