Matches in Nanopublications for { <http://purl.org/np/RA6U6VQH2TFco9whEYEq4SGF-xhT-qxATbkJ1QFblfOiE#step> ?p ?o ?g. }
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- step type Step assertion.
- step description "Seed 500 µl of cell suspension in a 24 well culture plate. Note: Monocytes in PBMCs get attached to the plastic in about 2-3 h when incubated at 37 °C. Longer incubation will result in firm attachement. Lymphocytes are not glass adherent and they will be mostly in suspension and can be removed by mildly flushing the wells with medium and/or buffer. Such treatment will keep the monocytes firmly attached to the surface of culture plates." assertion.
- step isStepOfPlan plan assertion.
- step isStepOfPlan plan assertion.
- step precedes step assertion.
- step precedes step assertion.
- step labEquipmentUsed _2 assertion.
- step bioSampleUsed _1 assertion.